[Cloud-Clone] Assay_Kits
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[Cloud-Clone] ELISA Kit for Versican (VCAN)
- Product No.: SEB817Mu
- Organism Species: Mus musculus (Mouse) Same name, Different species.
- Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test Method: Double-antibody Sandwich
- Assay Length: 3h
- Detection Range: 0.156-10ng/mL
- Sensitivity: The minimum detectable dose of this kit is typically less than 0.061ng/mL.
Specificity:
- This assay has high sensitivity and excellent specificity for detection of Versican (VCAN).
- No significant cross-reactivity or interference between Versican (VCAN) and analogues was observed.
Recovery:
Matrices listed below were spiked with certain level of recombinant Versican (VCAN) and the recovery rates were calculated by comparing the measured value to the expected amount of Versican (VCAN) in samples.
Matrix |
Recovery range (%) |
Average(%) |
serum(n=5) |
78-101 |
84 |
EDTA plasma(n=5) |
86-102 |
92 |
heparin plasma(n=5) |
79-98 |
91 |
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Versican (VCAN) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample |
1:2 |
1:4 |
1:8 |
1:16 |
serum(n=5) |
89-97% |
86-102% |
80-103% |
95-102% |
EDTA plasma(n=5) |
98-105% |
95-102% |
79-105% |
98-105% |
heparin plasma(n=5) |
81-91% |
87-98% |
88-101% |
93-101% |
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